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Advancements in next-generation sequencing and protein expression profiling have demonstrated that the renal targets of thiazides, i.e., Na + Cl − cotransporter (NCC, encoded by SLC12A3 ) and that of bumetanide/furosemide, i.e., Na + K + 2Cl – cotransporter-2 (NKCC2, encoded by SLC12A1 ) are expressed in different tissues and cells, albeit at lower levels (Di Fulvio and Alvarez-Leefmans, 2009)

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124 Dendrimer-based nanocarriers were also recently shown to penetrate full-thickness cartilage explants, and be retained in a native joint environment